High-speed image reconstruction for optically sectioned, super-resolution structured illumination microscopy

Super-resolution structured illumination microscopy (SR-SIM) is an outstanding method for visualizing the subcellular dynamics in living cells. To date, by using elaborately designed systems and algorithms, SR-SIM can achieve rapid, optically sectioned, SR observation with hundreds to thousands of time points. However, real-time still out reach most SIM setups as conventional algorithms image reconstruction involve a heavy computing burden. address this limitation, accelerated algorithm was developed implementing simplified workflow SR-SIM, termed joint space frequency reconstruction. This results 80-fold improvement speed relative widely used Wiener-SIM. Critically, increased processing does not come at expense spatial resolution or sectioning capability, demonstrated live imaging microtubule mitochondrial tubulation.